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Location:ホーム実験手法別製品・技術情報アッセイシステム

細胞内情報伝達物質アッセイシステム

代表的な測定マーカー: cAMP、cGMP、MAP Kinase、Protein Kinase C

製品 コード番号 原理 包装 アッセイレンジ 感度 操作時間 備考
cAMP[研究用] RPN3595 蛍光偏光 96 wells 1.32 〜 337.12 ng/ml
(4.0 〜 1,024 pmol/ml)

0.65 ng/ml

Overnight

  • Homogeneous method
  • Non-radioactive
  • Bright Cy3B-labelled probe
  • Includes lysis reagents that eliminate lengthy sample extraction procedures and allow direct cAMP measurement (optional use)
cAMP[研究用]

RPN3596

蛍光偏光 384 wells

3.29 〜 842.75 ng/ml
(10 〜 2,560 pmol/ml)

1.65 ng/ml

Overnight

  • Homogeneous method
  • Non-radioactive
  • Bright Cy3B-labelled probe
  • Includes lysis reagents that eliminate lengthy sample extraction procedures and allow direct cAMP measurement (optional use)
cAMP[研究用]
アセチル化タイプ
RPN225 EIA 96 wells

14 〜 840 pg/ml
(40 〜 2,500 fmol/ml)
[acetylation]

0.04 〜 10.52 ng/ml
(0.125 〜 32 pmol/ml)
[non-acetylation]

14 pg/ml
[acetylation]

38 pg/ml
[non-acetylation]

5.0 hr.
  • Now incorporates novel lysis reagents which eliminate lengthy sample extraction procedures and allow direct cAMP measurement (optional use)
  • Sample volume: 100 μl
cAMP [研究用]
非アセチル化タイプ
RPN2251
(1 kit)
EIA 96 wells 0.04 〜 10.52 ng/ml
(0.125 〜 32 pmol/ml)
38 pg/ml 5.0 hr.
  • Now incorporates novel lysis reagents which eliminate lengthy sample extraction procedures and allow direct cAMP measurement
  • Sample volume: 100 μl
  • No triethylamine is included
  • No acetic anhydride is included
cGMP RPN226 EIA 96 wells

14 〜 3,520 pg/ml
(0.04 〜 10.24 pmol/ml)
[acetylation]

0.175 〜 44.18 ng/ml
(0.5 〜 128 pmol/ml)
[non-acetylation]

14 pg/ml
[acetylation]

161 pg/ml
[non-acetylation]

5.0 hr.

1.5 days

  • Now incorporates novel lysis reagents which eliminate lengthy sample extraction procedures and allow direct cGMP measurement (optional use)
  • Sample volume: 100 μl
cGMP

TRK500

RIA[3H] 150 tubes

1.7 〜 27.6 ng/ml
(5 〜 80 pmol/ml)

140 pg/ml

3.5 hr.

  • Sample volume: 100 μl
cGMP

RPA525

RIA[125I] 100 tubes

0.175 〜 22.09 ng/ml
(0.5 〜 64 pmol/ml)
[non-acetylation]

7.0 〜 440 pg/ml
(20 〜 1,280 fmol/ml)
[acetylation]

175 pg/ml
[non-acetylation]

2.0 pg/ml
[acetylation]

1.5 days

  • Sample volume: 100 μl
IP3

TRK1000

RRA[3H] 100 tubes

0.8 〜 105 ng/ml
(1.9 〜 250 pmol/ml)

840 pg/ml

4.0 hr.

  • Sample volume: 100 μl
MAP Kinase

RPN84

Enzyme Assay 240 tubes

0.9 〜 27 pmol Pi/min (up to 10% incorporation of ATP)

at least 4.0 ng
(100 fmol)

1.5 hr.

  • Suitable for use with tissue / cell preparations
  • Sample volume: 15 μl

別途[γ-32P]ATP をご用意ください。

Protein Kinase C RPN77 Enzyme Assay 240 tubes

0.5 〜 10 pmol Pi/min
(up to 10% ATP incorporation)

2.8 ng/tube
(α/β/γ PKC purified from rat brain homogenate)

1.5 hr.
  • Suitable for use with tissue / cell preparations
  • Sample volume: 25 μl

別途[γ-32P]ATP をご用意ください。


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